This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems. •Provides researchers with solutions in lab environments •Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility •Information on a plethora of technologies needed to tackle complex problems